Dr. Rehab Mohammed Atta ElDesoukey graduated from the Faculty of Veterinary Medicine, Cairo University, Egypt, in 1997, earned a Master\'s in microbiology and immunology in 2004 and Doctorate completed in 2009 working as a researcher of Microbiology and Immunology at the National Research Center in Egypt, where he includes a selection of the greatest scientists and inventors in Egypt is also working as an assistant professor at Shaqraa university , Saudi Arabia .she is active ,has personal love of reading and explore everything new has conducted many researches in the field of microbiology that are looking for antimicrobials in everything new and strange .rn Abstract : One of the common problems in the medical world, spreading of bacterial resistance against antibiotics, Salvadora persica has biological active compounds and used in traditional medicine, it seems that this plant contain considerable antimicrobial capacity. So the aim of this study is to investigate the antimicrobial activity of Salvadora persica aqueous extracts on some medically important animal pathogens and to determine some phytochemical compounds. Aqueous extract of Salvadora persica were evaluated for their antimicrobial activity against some medically important pathogens isolated from animals and poultry farms. Also, phytochemical compound of aqueous extract was determined. So it could be concluded that the Salvadora persica extract Salvadora persica extracts contain tannins and saponin compounds and possess remarkable antimicrobial activity against microbial pathogens and to be introduced as an alternative to chemical antimicrobial drugs, is require!rn d wider investigation.rn
One of the common problems in the medicalrnworld, spreading of bacterial resistancernagainst antibiotics, Salvadora persica hasrnbiological active compounds and used inrntraditional medicine, it seems that this plantrncontain considerable antimicrobial capacity.
the aim of this study is to investigate thernantimicrobial activity of Salvadora persicarnaqueous extracts on some medicallyrnimportant animal pathogens and torndetermine some phytochemical compounds.
The aqueous extract is the most effectivernagainst Ps. aerogenes and B. cereusrnfollowed by Enterococcus , K. pneumoniarnand S.epidermis cold aqueous extractrnshowed significant antibacterial activityrnagainst, .While the ether extract did notrnshow any significant antibacterial S.rntyphimurium ,S.pyogens , and E. coli. Alsornthe extract showed high antifungal effectrnagainst C. albicans .
Walaa Mousa completed her BSc in Pharmaceutical Sciences from Mansoura University Egypt in 2005. Walaa completed her MSc in Biochemistry in 2010. In 2011, Walaa moved to Canada to study her PhD in Raizada lab at University of Guelph. Her current research involves: the development of new bacterial biocontrol agents to help control fungal pathogens of plants and elucidate the underlying mechanisms of antifungal activity.
Finger millet is an ancient African cereal crop, domesticated 7000 years ago in Ethiopia, reaching India at 3000 BC. Unlike other cereals, finger millet is resistant to the toxigenic fungal pathogen Fusarium graminearum. As this fungus is also ancient to Africa, we hypothesized that the crop may host beneficial endophytes (plant-colonizing microbes) that co-evolved to combat Fusarium. Here we describe the first ever report of endophytes from finger millet. We describe a novel Enterobacter species (strain M6). In vitro experiments demonstrate that strain M6 can inhibit the growth of Fusarium. When M6 was allowed to colonize the genetically related cereal crops, corn and wheat, which are susceptible to Fusarium, they acquired resistance to this pathogenic fungus, as demonstrated by replicated greenhouse trials. Confocal microscopy using GFP-tagged M6 showed that M6 colonizes the internal tissues of corn, wheat and millet and thus confirms that M6 behaves as an endophyte. To help understand the anti-Fusarium mechanism of action, M6 was co-cultured with Fusarium; microscopy using vitality stains demonstrated that M6 causes cleavage of fungal hypha at septa in vitro. To discover the anti-Fusarium genes, Tn5 mutagenesis followed by whole genome sequencing were conducted. Screening of 4800 Tn5 insertion events led to the discovery of 10 candidate genes/operons from M6. Expression of the candidate genes was studied by real time PCR, which showed that most of the genes are inducible by Fusarium. The importance of the Tn5 knockouts was confirmed in replicated greenhouse trials. The candidate anti-Fusarium genes from M6 include operons that encode phenazine (a potent anti-fungal metabolite), butanediol (an elicitor of host plant defences), and a fusaric acid resistance protein (FARP). Fusaric acid is a metabolite produced by Fusarium pathogen to inhibit the biosynthesis of bacterial phenazine; FARP biosynthesized by M6 bacteria is an apparent efflux transporter for fungal fusaric acid. Since both Fusarium and finger millet appear to be ancient to Africa, the phenazine-butanediol-fusaric acid-FARP interaction network may represent a fascinating example of three-way co-evolution between an endophyte, a pathogen and a host. It is hoped that modern agriculture will benefit from this ancient selection pressure in Africa.