Mostafa El-gaffary
Cairo University, Egypt
Title: In-vitro inhibition of BVDV as surrogate model for HCV using novel gold nanoparticles
Biography
Biography: Mostafa El-gaffary
Abstract
In this study, we evaluated in-vitro cytotoxic eff ect and antiviral properties of gold nanoparticles, which are previously r eported to possess in-vitro antiviral properties against HIV and multi strains of infl uenza virus. To investigate the antiviral activity of gold nanoparticles against cytopathic strain (NADL) bovine viral diarrhea virus (BVDV): citrated gold nanoparticles of 7±2 nm were prepared and PEG functionalized. Evaluation of the cytotoxicity of prepared gold nanoparticles did not show toxic eff ects to MDBK cells with concentration of 2 and 4 ppm. Aft erward the antiviral activity of nanoparticles was evaluated by the inhibition of the cytopathic eff ect on infected MDBK cells by means of (MTT) based colorimetric assay and was found that 4 PPM is the optimum concentration for virus inhibition. Th e results of the in- vitro antiviral activity and cytotoxicity showed that prepared gold nanoparticles has limited in-vitro toxic eff ect at concentration of 4 PPM also has strong affi nity to BVD virus and reasonable inhibitory eff ect on BVDV.
